human liver hepatocellular carcinoma hepg2 cells  (ATCC)

Journal: mSphere

Article Title: Plasmodium DNA ligase I is essential for parasite blood- and liver-stage development

doi: 10.1128/msphere.00674-25

Figure Lengend Snippet: Pb Lig1 is essential for parasite development in the liver. ( A ) HepG2 cells were infected with either UIS4/Flp or Pb Lig1 cKO sporozoites. Infected cultures were fixed at the indicated time points post-infection and immunostained with an anti-UIS4 antibody to visualize EEFs. ( B ) EEF area was comparable at 24 hpi ( P = 0.2500) and 36 hpi ( P = 0.1840), but was significantly reduced in Pb Lig1 cKO parasites at 55 hpi (**** P < 0.0001). Data represent the mean ± SEM from three independent experiments. ( C ) Quantification of EEF numbers at 24, 36, and 55 hpi revealed no significant differences between UIS4/Flp and Pb Lig1 cKO parasites (24 hpi, P = 0.6063; 36 hpi, P = 0.9036; 55 hpi, P = 0.9929; one-way ANOVA).

Article Snippet: Human liver hepatocellular carcinoma (HepG2) cells (ATCC) were used for in vitro infection of sporozoites.

Techniques: Infection

Journal: mSphere

Article Title: Plasmodium DNA ligase I is essential for parasite blood- and liver-stage development

doi: 10.1128/msphere.00674-25

Figure Lengend Snippet: Pb Lig1 is essential for nuclear division and merozoite development. ( A ) HepG2 cells infected with UIS4/Flp or Pb Lig1 cKO sporozoites were fixed at 62 hpi and immunostained with anti-UIS4 and anti-MSP1 antibodies. UIS4/Flp parasites exhibited robust MSP1 staining, indicative of merozoite formation, whereas Pb Lig1 cKO parasites lacked MSP1 signal, suggesting a block in merozoite development. ( B ) Quantification of nuclear number at 55 hpi. Nuclei were manually counted in ImageJ-analyzed images. Pb Lig1 cKO EEFs displayed a significantly reduced number of nuclei compared to UIS4/Flp (**** P < 0.0001, unpaired t -test). ( C ) Quantification of DNA content using Hoechst 33342 staining at 36 hpi and 55 hpi. UIS4/Flp and Pb Lig1 cKO EEFs were fixed and immunostained with anti-UIS4 and anti-MSP1 antibodies. Nuclear DNA within the PV was quantified by calculating corrected total cell fluorescence (CTCF) from Hoechst signal. Data represent 43 (UIS4/Flp 36 hpi), 47 ( Pb Lig1 cKO 36 hpi), 60 (UIS4/Flp 55 hpi), and 60 ( Pb Lig1 cKO 55 hpi) individual EEFs. No significant difference in CTCF was observed at 36 hpi ( P = 0.0693), while a significant reduction was detected in Pb Lig1 cKO parasites at 55 hpi (**** P < 0.0001, unpaired t -test), n.s., not significant. Data are shown as mean ± SD from two independent biological replicates.

Article Snippet: Human liver hepatocellular carcinoma (HepG2) cells (ATCC) were used for in vitro infection of sporozoites.

Techniques: Infection, Staining, Blocking Assay, Fluorescence

Journal: Discover Oncology

Article Title: Isosilybin B: a potential novel therapeutic agent with hepatoprotective, anticancer and antifibrotic properties

doi: 10.1007/s12672-025-03380-8

Figure Lengend Snippet: Effect of IB/SB/SM on cytotoxicity and cell cycle arrest of liver cells. A : Chemical structure of IB and SB (silybin A and silybin B in ratio 1:1). Cytotoxicity in Hepa1-6 ( B ), HepG2 ( C ) or AML12 ( D ) liver cells after 24 h treatment with IB/SB/SM. Changes in the cell cycle in Hepa1-6 ( E ), HepG2 ( F ), or AML12 ( G ) liver cells after 24 h treatment with 31.3 µg/mL of IB/SB/SM. H : Cell distribution of diploid cells in the phases of the cell cycle shown for IB (31.3 µg/mL). The data are given as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Human liver hepatocellular carcinoma cell line HepG2 (RRID: CVCL_0027, ATCC, USA) was cultivated in RPMI medium supplemented with 10% FBS and 1% penicillin-streptomycin.

Techniques: